Availability limited to KIT internal users (starting 25th call)
Matrix assisted laser desorption ionization (MALDI) or electrospray ionization (ESI) allows the soft ionization and transfer of analytes to the gas phase. Time-of-flight (TOF) mass spectrometric analysis provides high-resolution, exact mass measurement and accurate isotope distributions of positive or negative ions for identification. This allows for chemical identification of intact molecular and cluster species, transferred from solution (ESI) or a solid matrix (MALDI).
Recent advances in technology have led to the efficient coupling of ion-mobility analysis with high resolution mass spectrometers. Ion mobility spectrometry (IMS) provides a means to separate ions based on their shape and size, providing complementary information to that obtained via standard mass spectrometry. The Synapt-G2 HDMS is the first commercial instrument of its kind, offering flexibility in terms of ion source (MALDI, nanoESI, ESI) and sample analysis, coupling a high resolution TOF mass analyzer with a travelling wave ion mobility (TWIMS) separation cell. Thus, the instrument has both the capability to obtain high resolution ESI-/MALDI-TOF mass spectra, or, with application of IMS, to obtain 2D structure-mass correlation maps. Ions of interest may also be mass selected in a quadrupole mass filter and have their individual chemistry (e.g. via collision induced dissociation) probed.